Azospirillum (AZO) and Gluconacetobacter (GLU) are two genera of naturally occurring bacteria that convert nitrogen, a vital plant nutrient, from its atmospheric form into a form usable by plants. AZO is associative and functions around the plant roots, whereas GLU is endophytic, working within the plant tissue. Nitrogen-fixing bacteria are gaining popularity in sustainable agriculture efforts as biofertilizers to provide an alternative to environmentally problematic synthetic fertilizers. Companies such as TerraMax Inc. are interested in finding the most effective bacteria for biofertilizers to optimize crop yield. This research compared the impact on corn growth and function of the more widely used AZO and the novel biofertilizer candidate GLU, both with varying application methods: soil drench, leaf coating, or a combination of those two. The results favor GLU over AZO for increasing nitrogen availability in corn and indicate the potential for GLU to improve yield through significantly increasing leaf net photosynthesis, chlorophyll content, and shoot biomass compared to the control when applied directly to the leaves. These results suggest Gluconacetobacter (GLU) as a promising bacterial candidate for companies like TerraMax Inc. seeking to improve biofertilizer performance, ultimately reducing the need for synthetic fertilizers and promoting environmental sustainability.
