Antibiotic shortages in tandem with the rise of antibiotic resistance pose significant global public health threats. These obstacles emphasize the importance for continued research and support in the search for novel antibiotic-producing bacteria that carry new lines of defense against human pathogens. Soil contains a biodiverse community of microbial species with an extensive range of antibiotic producing pathways. In nutrient-poor soils, competition amongst microbes is enhanced driving the evolution of unique antimicrobial compounds that may be used in the production of novel antibiotics. This study sought to isolate and characterize antibiotic-producing bacteria from nutrient-poor soils. Soil samples were extracted using a core from three sites. The samples were plated to observe microbial growth. Unique colonies varying in morphology, pigmentation, and size were identified and transferred to a separate plate. The unique colonies were tested for antimicrobial activity against four pathogens: Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Salmonella typhimurium. Colonies that inhibited the growth of the pathogenic strains were isolated and characterized using the Gram stain procedure. Across the three sites 23 isolates were obtained. The isolates showed resistance to E. coli, S. aureus, and B.subtilis. Further genetic testing will be needed to taxonomically identify novel antibiotic-producing bacteria.
