Fragile X syndrome (FXS) is the most common cause of inherited intellectual disability and the leading genetic cause of autism spectrum disorder. FXS is caused by loss-of-function mutations of the FMR1 gene; how FMR1 loss affects neurodevelopment is still being characterized. Our objective was to assess how the FXS genotype affects swimming behavior development in wildtype zebrafish vs fmr1 knockout zebrafish. Zebrafish larvae of wildtype and fmr1 knockout backgrounds were assessed at 5 days post fertilization for distance moved, time spent moving, and velocity over an alternating light-dark cycle using DanioVision hardware with EthoVision XT software (Noldus). RStudio was used for performing Kruskal-Wallis tests to test significant differences between means. Wildtype zebrafish and fmr1 knockout zebrafish had similar swimming patterns in response to alternating light-dark exposure. These results indicate that fmr1 does not regulate swimming behavior development in larval zebrafish.
