Overuse of antibiotics in agriculture and healthcare settings results in increased microbial resistance to antibiotics. In order to maintain an advantage in treating disease caused by microorganisms, we must discover and develop new antibiotics and minimize misuse. This in turn creates difficulty in treating diseases that were previously treatable with antibiotics. In previous studies we surveyed 4 different farm soils and discovered many antibiotic-resistant bacteria. In this study we used the same soil samples to determine the incidence of antibiotic-secreting bacteria across WI and MN. We tested resistance to gram-positive and gram-negative bacteria. We discovered 24 isolates that produced antimicrobial effects against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Out of the 8 isolated strains, we found 4 strains that inhibited both E. coli and S. aureus, 3 strains inhibited only S. aureus, and 1 isolate was only effective against E. coli. Preliminary characterization of the isolates indicates that we have 7 gram-positive rods and 1 gram-negative rod. We are in the process of further characterizing strains and identification of antimicrobial properties.
