Understanding the cow rumen microbiome is an ongoing project with significant implications for agriculture, as the health, weight, and methane emissions of the animal are tied to the microbiome. However, knowledge of rumen microbiomes is biased towards dairy cows and geographically influenced by European breeds. Therefore, to more comprehensively understand the contributions of the microbiome to sustainable animal agriculture, there is a need to study American and beef cattle rumen microbial communities. Using metagenomic techniques, we identified 1,329 microbial genomes from beef cattle rumen fluid. Using the Blugold HPC, we compared these genomes to a database of 12,906 microbial genomes compiled from different ruminants to determine which were newly-sampled. This identified 505 rumen microbial genomes that were uniquely-recovered in our American beef cattle metagenomes. We selected a genome classified as a Prevotella, a ubiquitous rumen genus, and characterized its phylogeny, revealing it likely represents a novel species. We will characterize its metabolic potential to understand the role of this genome in rumen microbiome carbon and nitrogen cycling. This work will lead to a more thorough understanding of the rumen microbiome, informing any efforts to improve animal health, reduce methane emissions, and otherwise improve farming practices.
